Question by ☆♥Angelic Voice♥☆: How is the Bt gene introduced into the genetic code of plants?
My friend at a diverse college was curious. Sorry about the extended question-also if you can answer this: How is Bt used differently for organic farming than in GM corn?
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Answer by john h
1st, scientists recognize a strain of Bt that kills the targeted insect. Then they isolate the gene that produces the lethal protein. That gene is removed from the Bt bacterium and a gene conferring resistance to a chemical (generally antibiotic or herbicide) is attached that will prove useful in a later step.
The Bt gene with the resistance gene attached is inserted into plant cells. At this point, scientists must figure out which plant cells have effectively received the Bt gene and are now transformed. Any plant cell that has the Bt gene must also have the resistance gene that was attached to it. Researchers grow the plant cells in the presence of the antibiotic or herbicide and pick the plant cells that are unaffected by it. These genetically transformed plant cells are then grown into entire plants by a procedure known as tissue culture. The modified plants generate the very same lethal Bt protein created by Bt bacteria due to the fact the plants now have the very same gene.
Organic farming use the Bt Bacteria to manage insects use precisely the very same the same lethal Bt protein as the genetically modified plants because they have the exact same gene.
http://www.biotech.iastate.edu/biotech_info_series/bio9.html
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